OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION OF POLYPHENOLS FROM HONEYSUCKLE (Lonicera caprifolium)
Article Sidebar
Main Article Content
Abstract
Honeysuckle (Lonicera caprifolium) is a perennial flowering plant from the genus Lonicera of the family Caprifoliaceae. Lonicera caprifolium flowers are used in herbal traditional medicine for their antibacterial, antioxidant and antiviral activities. The objective of this research was to optimize the ultrasound-assisted extraction (UAE) process of polyphenols from Lonicera caprifolium flowers using Design of Experiments (DoE) and Response Surface Methodology (RSM). We used a three-level factorial model with three variable factors (ethanol concentration, time and the ratio between solvent and plant material). The model and the data indicated that the ethanol concentration was highly significant (p<0.05), while the other two were less significant for the extraction of the maximum total phenolic compounds and the maximum antioxidant activity of the extracts. The antioxidant activity was performed by DPPH, ABTS and FRAP methods. The polyphenols were identified by HPLC. The optimal condition obtained for the extraction of polyphenols from honeysuckle by UAE will be used for bioproduct formulation in future work.