SELECTING THE OPTIMAL CONCENTRATION OF NUTRIENTS, DISPERSANT AND DIESEL OIL TO ENHANCE METABOLIC ACTIVITY AND DIESEL OIL CONSUMPTION BY MARINE ENDOGENOUS MICROBIOTA AT 15 °C
Published in AgroLife Scientific Journal, Volume 3, Number 1
Written by Mihaela MANEA, Marinela CIRNU, Ioan ARDELEAN
In this paper we present our results concerning further screening for optimal concentration of dispersant (Nacol C), nutrients and diesel oil to sustain metabolic activity of marine autochthonous microbiotain order to enhance the biological consumption of diesel oil. The results show that the overall metabolic activity (reported as ng reduced resazurine/hour/wheel), is decreased by diesel addition (10% final “concentration”) either in the absence or in the presence of dispersant (Nacol C 1/10.000 dilution); inorganic nutrients additions (both ammonium acetate and ammonium phosphate 0.5%) greatly (4 to 10 times) increase the rate of resazurine reduction whereas further organic nutrient addition sustains an even higher rate of resazurine reduction. Diesel oil consumption is 1.5 times enhanced by dispersant addition but organic and/or inorganic nutrients additions do not have a further effect on diesel consumption, the values obtained being within the level of standard deviations.