ANTIOXIDATIVE PROPERTIES OF Plantago lanceolata L. EXTRACTS EVALUATED BY CHEMILUMINESCENCE METHOD

Published in AgroLife Scientific Journal, Volume 5, Number 2
Written by Cornelia NICHITA, Georgeta NEAGU, Ana CUCU, Virginia VULTURESCU, Șerban Vifor Gabriel BERTEŞTEANU

Plantago lanceolata L. is used as astringent, anti-inflammatory, immunoregulatory, antispasmodic, expectorant, emollient, topical anodyne, antibacterial for cuts, bruises and abscesses. These pharmacological properties are due to its complex chemical composition: iridoid glycosides, polysaccharides (plantaglucide, glucomannon), terpenoids, coumarins, lignans, caffeic acid derivatives, polysaccharides, flavonoids, flavone glycosides and other constituents, like allantoin. In this paper, antioxidant activity, qualitative and quantitative determination of some active components (flavonoids, polyphenols, and polyphenols carboxylic acids) are evaluated, taking into account the biological properties. Antioxidant properties were evaluated by chemiluminescence (CL) technique in system generator based at 5-amino-2.3-dihydrophthalazine-1.4-dione and hydrogen peroxide, in alkaline medium and DPPH (2.2-diphenyl-1-picrylhydrazyl) method. The total flavonoids, polyphenols content and polyphenol carboxylic acids was spectrophotometrically determined according to Romanian Pharmacopoeia (FR). In addition, the qualitative biochemical fingerprint was investigated by mass spectrometry LC-ESI-MS. The mass spectral data and amount of active components (flavonoids, polyphenols, polyphenols carboxylic acids) demonstrated the chemical structural complexity. The results of the chemiluminescence in vitro tests revealed that the Plantago lanceolata L. extracts exhibit high antioxidant activity (84-96%), being in accordance with the value provided by DPPH free radical scavenging assay. Also is established a dependence directly proportional to the content of polyphenolic compounds found in herbal extracts with level of antioxidant activity.

[Read full article] [Citation]

NICHITA C., NEAGU G., CUCU A., VULTURESCU V., BERTEŞTEANU S.V.G. 2016, ANTIOXIDATIVE PROPERTIES OF Plantago lanceolata L. EXTRACTS EVALUATED BY CHEMILUMINESCENCE METHOD. AgroLife Scientific Journal, Volume 5, Number 2, ISSN 2285-5718, 95-102.


The publisher is not responsible for the opinions published in the Volume. They represent the authors’ point of view.
© 2015 AgroLife Scientific Journal. All Rights Reserved. To be cited: AgroLife Scientific Journal
Powered by INTEL TECH DEVELOPMENT.