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TRACING THE DEVELOPMENT OF Bacillus subtilis IN BROTH WITH ADDED MICELLES AND EXTRACTS FROM HIGHER MEDICINAL MUSHROOMS

Published in AgroLife Scientific Journal, Volume 10, Number 1
Written by Kamelia LOGINOVSKA, Maria DONEVA, Nikolay SOLAKOV, Alexander VALCHKOV, Iliana NACHEVA

The growth of cell culture of strain Bacillus subtilis NBIMCC 2353 has been traced by two experiments. At 48th hour the number of cells reaches its maximum, which is above 8 log units (experiment 1), after included on the 24th hour of enrichment agents in native form - mushroom body and micelles of the high medical mushrooms Ganoderma lucidum (Red reishi), and Cordyceps sinensis at concentrations of 2% and 4%. Cell culture basically fails to develop in its maximum capabilities due to difficulty absorbing macro-, micro-elements and protein compounds, contained in the high mushrooms. Positive effects have been found in experiment 2, such as prolonging the propagation time and doubling the number of cells of Bacillus subtilis NBIMCC 2353, upon added into the nutrient medium of easily accessible components, such as Cordyceps sinensis and Ganoderma lucidum powder extracts. The largest increase in cell amount was observed at the 54th hour from the start of incubation, which was over 11.5 log units, for variants with added 2 g extract

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