CAUSES AND SOLUTIONS FOR THE REMEDIATION OF THE POOR ALLOCATION OF P AND K TO WHEAT CROPS IN ROMANIA

Published in AgroLife Scientific Journal, Volume 5, Number 1
Written by Florin SALA, Ciprian RUJESCU, Cristian CONSTANTINESCU

The aim of the present study was to establish the main cause of the poor allocation of PK fertilizers to wheat crops, as this phenomenon has been observed in Romania. The experimental research consisted in differentiated allocation of fertilizers with N and the PK complex in order to create controlled nutrition deficits. It was conducted within the Didactic Station in Timisoara, from 2012 to 2014. Based on the yields obtained and on the associated economic elements, two scenarios were used: variation of wheat selling price and the price of fertilizers; each scenario had several variants. For each variant, the study assessed the optimal dose of N and PK respectively that resulted in the maximum profit. The model employed for this purpose was a model given by the production function verified by experimental data. The results were analysed and processed with SPSS and the graphic representations with Wolfram Alpha. The study discovered that the main cause of the Romanian farmers’ low interest in using PK fertilizers is the dissonance between the cost of PK fertilizers and the low market price of wheat. Given the current price of wheat - between 0.5 - 0.6 lei/ kg-1, the authorities have to adopt incentive pricing for PK fertilizers in order to stimulate the use of such fertilizers by Romanian farmers. These measures will result not only in better quality and quantity of the yield, but also in medium and long-term improvement of soil fertility.

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SALA F., RUJESCU C., CONSTANTINESCU C. 2016, CAUSES AND SOLUTIONS FOR THE REMEDIATION OF THE POOR ALLOCATION OF P AND K TO WHEAT CROPS IN ROMANIA. AgroLife Scientific Journal, Volume 5, Number 1, ISSN 2285-5718, 184-193.

CHEMICAL DIVERSITY OF POLYPHENOLS FROM BEE POLLEN AND PROPOLIS

Published in AgroLife Scientific Journal, Volume 6, Number 2
Written by Roxana SPULBER, Teodora COLȚA, Narcisa BĂBEANU, Ovidiu POPA

Bee pollen and propolis represent the bee products with the highest concentration of phenolic substances. These products contains a variety of chemical compounds but phenolic compounds are considered the main bioactive constituents. The chemical composition in both cases varies with botanical sources available to bees and extraction conditions. Despite chemical diversity, most of the samples share a similar phenolic profile. Only a part of phenolic constituents like flavonoid glycosides and pigments have been demonstrated the use as markers to discriminate bee pollen or propolis from different botanical origins.This paper highlights the main factors that contributes to chemical diversity of polyphenols from bee pollen and propolis.

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SPULBER R., COLȚA T., BĂBEANU N., POPA O. 2017, CHEMICAL DIVERSITY OF POLYPHENOLS FROM BEE POLLEN AND PROPOLIS. AgroLife Scientific Journal, Volume 6, Number 2, ISSN 2285-5718, 183-194.

Classification of genus Triticum sensu lato and sensu stricto based on spike and grain morphology

Published in Scientific Papers. Series "AgroLife Journal", Vol. 4 ISSUE 1
Written by Hristo P. STOYANOV

The formulation of the present classifications of species of the genus Triticum associates mainly with several plant morphological factors such as fragility of the spikes spindle, grains threshability, grain sphericity, shape and position of glumes, lemmas and paleas and awns, compactness, etc. Special attention is paid to the factor "cultural/wild" form, the ploidy and the genomic constitution of the species, often supported by molecular data which provides considerable comfort in disclosing phylogenetic features in a particular taxonomic unit. Such taxonomic determination is associated with certain disadvantages. It is not sufficiently focused on the spike morphology related to the reproductive apparatus of the plant, and also the causes of phylogenetic differentiation of certain parameters, such as spike branching, multiple spikelets, as well as the ratios of quantitative properties. The existing classifications do not give a precise answer to the taxonomic position of amphidiploids in the genus Triticum, and also for those obtained from hybrid combinations with genera Aegilops, Secale, Haynaldia, Hordeum, Elymus, Leymus, Elytrigia, Agropyron, as transitional and similar forms. Based on studies of spike and grain morphology of a large number of representatives of the genus Triticum and other interspecific and intergeneric amphidiploid forms, a classification of the genus sensu lato and sensu stricto is composed. Sensu stricto, genus Triticum covers all existing wild and cultivated known wheat forms, together with interspecific artificial synthetic forms. Sensu lato, the genus includes intergeneric hybrids, for which a specific generic epithet was coined - ×Triticum, and also a specific epithet, consistent with the originator of the amphidiploid. Special attention was paid to species and amphidiploids with the genus Aegilops. Classification sensu strictissimo was also formulated where the genus Triticum brings together only diploid species, but natural amphidiploids are separated as genus Aegilotriticum, and artificial as genus ×Aegilotriticum, and the remote intergeneric amphidiploids are not subject to the classification. Drawn up in this way, the classifications cover morphological and ecological, evolutionary and phylogenetic features of the representatives of the genus Triticum

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Hristo P. STOYANOV 2015, Classification of genus Triticum, sensu lato and sensu stricto, based on spike and grain morphology. Scientific Papers. Series "AgroLife Journal", Vol. 4 ISSUE 1, PRINT ISSN 2285-5718, 176-185.

CLIMATE CHANGE TRENDS IN SOME ROMANIAN VITICULTURAL CENTERS

Published in AgroLife Scientific Journal, Volume 5, Number 2
Written by Georgeta Mihaela BUCUR, Liviu DEJEU

The evolution of temperature indicators, more pronounced in the north of Romania, clearly shows a gradual warming trend, mainly during the growing season, especially during the ripening of the grapes. There have been highlighted relatively large differences between the values of the main bioclimatic indicators of the country's wine regions, as well as a stronger growth trend of these ones in the northern areas and at the Black Sea. The viticultural region of Dobrogea hills profits by maritime influences that limit summer temperature extremes and winter frost. There is evidence that in many regions, day temperature increased more than night temperature. Results show that Romanian viticulture will benefit from climate change. Given these trends and knowing their possible negative effects on vine physiology, production and quality, the future viticultural zoning requires reconsideration, and the application of technological measures to minimize the negative effects of climate change.

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BUCUR G.M., DEJEU L. 2016, CLIMATE CHANGE TRENDS IN SOME ROMANIAN VITICULTURAL CENTERS. AgroLife Scientific Journal, Volume 5, Number 2, ISSN 2285-5718, 24-27.

CLINICAL AND PARACLINICAL PECULIARITIES OF EFFUSIVE FELINE INFECTIOUS PERITONITIS UNDER NON-SPECIFIC IGY TREATMENT: CASE STUDY

Published in AgroLife Scientific Journal, Volume 6, Number 1
Written by Teodora Diana SUPEANU, Alexandru SUPEANU, Dragoş COBZARIU, Stelian BĂRĂITĂREANU, Doina DANEŞ

Feline infectious peritonitisis a disease determined by a coronavirus, affectingdomestic and wild cats and once triggered is fatal in almost 100%. The occurrence of the related pathology are yet to be fully determined; currently, a certain and confident in-clinicaldiagnosis is unavailable, as is the case for an efficient vaccine or a treatment that will restore to health. These make the feline infectious peritonitis a subject currently undergoing intensive research studies. The paper presents the case of a 5 months cat patient manifesting the rapid evolution effusive form of the disease. The aim of study was to evaluate if the variation of the 24 biological parameters studied was significant under treatment with immunoglobulins of avian origin. The patient tolerated well the supportive therapy based on egg yolk Immunoglobulin Y, without evidences of side effects.

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SUPEANU T.D., SUPEANU A., COBZARIU D., BĂRĂITĂREANU S., DANEŞ D. 2017, CLINICAL AND PARACLINICAL PECULIARITIES OF EFFUSIVE FELINE INFECTIOUS PERITONITIS UNDER NON-SPECIFIC IGY TREATMENT: CASE STUDY. AgroLife Scientific Journal, Volume 6, Number 1, ISSN 2285-5718, 257-261.

CLINICAL AND ULTRASOUND FEATURES IN DYSURIC DOGS

Published in AgroLife Scientific Journal, Volume 6, Number 2
Written by Mario CODREANU, Alexandra Mihaela POPA, Iuliana CODREANU, Raluca NAE

This article briefly indicates basic definitions and determining causes (neurogenic and non-neurogenic). The survey was accomplished in Faculty of Veterinary Medicine, Bucharest, on a total of 25 dogs between octomber 2016 and february 2017. Ultrasound was performed using Esaote Pie Medical. The clinical signs that drew attention, were dominated by pollakiurie, stranguria (associated with the phenomena of bladder stones and the obstructive urethral, especially in males) and hematuria (recurrent haematuria, and/or persistent, appeared in lithiasic condition). Urinary retention has been shown to be, in most cases (62.06%) of neurogenic origin. Proliferative parietal modifications, were consecutive to neoplasia, in form of formations tumor type pedicle, and type diffuse infiltrative. According to our study, most common bladder disorders detected in the ultrasound examination were the inflammatory type, especially the acute form, determined, in a large proportion by microlithiasis and macrolithiasis.

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CODREANU M., POPA A.M., CODREANU I., NAE R. 2017, CLINICAL AND ULTRASOUND FEATURES IN DYSURIC DOGS. AgroLife Scientific Journal, Volume 6, Number 2, ISSN 2285-5718, 67-72.

Coliform bacteria contamination profiling of raw milk cheeses using statistical assessment of typelinked correlations

Published in AgroLife Scientific Journal, Volume 3, Number 2
Written by Mara GEORGESCU, Constantin SAVU, Iuliana NEAGU, Dragos GEORGESCU

In the light of the current trend of increasing consumption of ecological, unprocessed and slow food, hygiene-associated microbiological drawbacks of raw milk cheeses are still an issue. This study was designed for the coliforms contamination assessment of multiple types of raw milk cheeses manufactured by local producers. The paper is focused on searching for possible correlations between the sample type and coliform bacteria contamination, using the chisquared test of significance for statistical processing of the data. The results for a total sample size of 253 cheeses indicated significant associations (p<0.0005) between salting, smoking, milk origin and cheese recipe, on one hand and coliform bacteria contamination on the other hand. No significant association (p=0.438) was found between coliforms and whey-cheese versus milk-cheese classification parameter.

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Mara GEORGESCU, Constantin SAVU, Iuliana NEAGU, Dragos GEORGESCU 2014, Coliform bacteria contamination profiling of raw milk cheeses using statistical assessment of typelinked correlations. AgroLife Scientific Journal, Volume 3, Number 2, ISSN 2285-5718, 35-38.

COMPARATIVE STUDY OF IN VITRO, EX VITRO AND IN VIVO PROPAGATED Salvia hispanica (CHIA) PLANTS: MORPHOMETRIC ANALYSIS AND ANTIOXIDANT ACTIVITY

Published in AgroLife Scientific Journal, Volume 5, Number 2
Written by Ely ZAYOVA, Milena NIKOLOVA, Ludmila DIMITROVA, Maria PETROVA

A tissue culture technique for micropropagation of chia (Salvia hispanica L.) was established. High percentage of seed germination (100%) was recorded on Murashige and Shoog medium enriched with 0.4 mg l-1 gibberellic acid and 10 mg l-1 ascorbic acid after one week of culture. The maximum number of shoots per explant (2.7) gave MS medium with 2 mg l-1 6-benzylaminopurine after five weeks of culture. The best plant rooting was achieved on half-strength MS medium with 0.1 mg l-1 indole-3-butyric acid after four weeks of culture. The multiple plants were successfully ex vitro adapted with 95% survival. After six months under field conditions, important morphometric traits of ex vitro and in vivo derived plants were evaluated. Some morphological characteristics as plant height, leaf size, number of branches and leaves per plant were influenced by the propagation way. Differences between extracts of in vitro, ex vitro and in vivo developed plants in regard to free radical scavenging activity, flavonoid and lipid composition were not established. This is the first comparative study of micropropagated and in vivo seed derived chia plants. The study presents also for the first time data for antioxidant potential and lipid components of leaves of S. hispanica.

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ZAYOVA E., NIKOLOVA M., DIMITROVA L., PETROVA M. 2016, COMPARATIVE STUDY OF IN VITRO, EX VITRO AND IN VIVO PROPAGATED Salvia hispanica (CHIA) PLANTS: MORPHOMETRIC ANALYSIS AND ANTIOXIDANT ACTIVITY. AgroLife Scientific Journal, Volume 5, Number 2, ISSN 2285-5718, 166-174.

COMPARISON OF FOUR GENOMIC DNA ISOLATION METHODS FROM SINGLE DRY SEED OF WHEAT, BARLEY AND RYE

Published in AgroLife Scientific Journal, Volume 6, Number 1
Written by Daniel CRISTINA, Matilda CIUCĂ, Călina-Petruța CORNEA

Modern breeding programs are based on the differences that distinguish one plant from another one, differences encoded in the plant’s genetic material, the DNA. Genotypic selection, particularly at the DNA level, can be exploited in Marker Assisted Selection (MAS) to identify desirable recombinants among segregating populations. Successful DNA amplification is vital for the detection of specific DNA targets, and this depends on the ability of DNA isolation methods to produce good quality DNA. DNA isolation from plant tissues remains difficult because of the presence of a rigid cell wall surrounding the plant cells. DNA isolation methods are affected by several factors like the amount of tissue needed and its availability, the number of steps involved and the chemicals used. In this study four different DNA isolation methods, based on CTAB and SDS (three methods), applied on single dry seed of wheat, barley and rye, were tested and compared. The quality of DNA was assessed by spectrophotometric measurements, gel electrophoresis and PCR reactions. The results of the experiments showed that DNA isolated by all four isolation methods is not excessively fragmented, A260/A280 ratio was between 1.6-1.9 and the concentration ranged between 20-194 ng/μl. The quality of the DNA was good and allowed the amplification of specific fragments by PCR. CTAB method had better A260/A280 ratio followed by SDS1, SDS2 and SDS3. Electrophoretic pattern showed better results with SDS2 method, followed by SDS3, SDS1 and CTAB. Furthermore, the CTAB and SDS1 methods need more time than SDS2 and SDS3, even though the number of steps of each method are almost equal (±2 steps). An estimative cost per sample showed that the cheapest method is the SDS3, the other three having similar costs.

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CRISTINA D., CIUCĂ M., CORNEA C.P. 2017, COMPARISON OF FOUR GENOMIC DNA ISOLATION METHODS FROM SINGLE DRY SEED OF WHEAT, BARLEY AND RYE. AgroLife Scientific Journal, Volume 6, Number 1, ISSN 2285-5718, 84-91.

CORRELATIONS BETWEEN SOMATIC CELL COUNT AND MILK QUALITY IN TWO DAIRY FARMS FROM NORTH-WESTERN ROMANIA

Published in AgroLife Scientific Journal, Volume 6, Number 1
Written by Iosif SARAZ, Mihai CENARIU, Simona CIUPE, Raul POP, Ioan GROZA

The objectives of this study were to achieve an epidemiological investigation on subclinical mastitis in dairy cows and to evaluate milk quality according to factors such as somatic cell number, protein, fat, dry matter, freezing point. The study was carried out during 2014 in two farms located in north-west of Romania. Detection of subclinical mastitis at farm A was carried out using MAST-O-TEST method and at farm B by KERBATEST method. In terms of temporal evolution, the highest incidence of subclinical mastitis was recorded in summer (farm A: 4.38%, 5.18%, 5.78% and farm B: 4.46%, 5.36%). Regarding subclinical mastitis incidence in relation to age of the animals, the highest values were observed in animals ranging from 4-6 years (62.75% - farm A and 60.00% - farm B). The epidemiological study conducted in relation to the stage of lactation showed that the highest rate was recorded in cows in the 4th lactation (47.55% - farm A and 48.57% - farm B). The overall analysis of the results relating to the anatomical positioning of the affected quarter revealed that the highest frequency was observed in the hindquarters (68.16% - farm A and 68.57% - farm B). A higher incidence of subclinical mastitis was observed during summer season and therefore biochemical analysis and determination of somatic cells number (SCC) were performed only during this period. For these determinations an automated system - CombiFoss was used. The control group (C) showed SCC values between 130 - 300 SCCx10-3 mL-1 while in mastitis group (M) the number of somatic cells was between 678 - 1,705 SCCx10-3 mL-1. The mean values for the other parameters were as follows: dry matter (C-10.18%, S-8.36%), protein (C-3.41%, S- 2.88%), fat (C-4.06%, S-3.28%), freezing point (C-0.529, S-0.609).

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SARAZ I., CENARIU M., CIUPE S., POP R., GROZA I. 2017, CORRELATIONS BETWEEN SOMATIC CELL COUNT AND MILK QUALITY IN TWO DAIRY FARMS FROM NORTH-WESTERN ROMANIA. AgroLife Scientific Journal, Volume 6, Number 1, ISSN 2285-5718, 234-236.


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