EFFECT OF CRUDE FIBRE CONCENTRATION IN PULLET DIETS (9-16 WEEKS) ON THEIR SUBSEQUENT PERFORMANCE

Published in AgroLife Scientific Journal, Volume 5, Number 1
Written by Cristinel Vasile PANAITE, Rodica Diana CRISTE, Dumitru DRAGOTOIU, Tatiana Dumitra PANAITE, Margareta OLTEANU

The EU produces more than 190 million tons of meat, milk and eggs. About 450 million tons of feeds/year are needed to support this production in the 28 EU member states. Research and innovation in animal feeding and in feed manufacturing technology are basic for the support of animal production in the EU, being the most profitable investment for this industrial sector. One of the problems with market demands regards the economic efficiency of the production, which involves diet formulations and choosing feed ingredients. Fibre-high feed ingredients can decrease the cost of feeding because of their lower costs compared to the conventional raw materials used in poultry diets. The paper reports the findings of a study on the effect of higher dietary crude fibre levels given to replacement pullets on their subsequent performance. A feeding trial was conducted on 240 Lohmann Brown pullets (9-16 weeks). The pullets have been assigned randomly to four groups (C, E1, E2, and E3), with 60 pullets per group, housed in an experimental hall under controlled environmental conditions. The diets for the four groups were characterized by 14.5% CP and 2800 kcal/kg ME. Compared to the diet for group C (5% fibre/kg compound feed, CF), the experimental diets had different levels of dietary fibre, given by the different amounts of dietary alfalfa: 6% fibre/kg CF (E1), 8% fibre/kg CF (E2), and 10% fibre/kg CF (E3), in combination with 2 enzymatic products that improve fibre digestibility (Biozim M6000 and Digestarom). The production parameters (feed intake, body weight, weight gain and animal welfare) were monitored throughout the experimental period. The experimental results have shown that feeding pullets (9-16 weeks) with compound feeds high in fibre (6% - E1; 8% - E2 and 10% - E3), didn’t affect the growth parameters. The positive results compared to group C were recorded for group E2 (8% dietary fibre). The average live weight of E2 pullets (1419.5±97.76g GV) was significantly (P≤0.5) higher (by 4.65%) compared to group C pullets (1356.33±110.64 g LW). The live weight of E2 pullets also was 6.73% higher compared to the values stated in the Lohmann Brown management guide (1330.00 g LW).

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PANAITE C.V., CRISTE R.D., DRAGOTOIU D., PANAITE T.D., OLTEANU M. 2016, EFFECT OF CRUDE FIBRE CONCENTRATION IN PULLET DIETS (9-16 WEEKS) ON THEIR SUBSEQUENT PERFORMANCE. AgroLife Scientific Journal, Volume 5, Number 1, ISSN 2285-5718, 161-167.

EFFECT OF OREGANO AND ROSEHIP SUPPLEMENTSON BROILER (14-35 DAYS) PERFORMANCE, CARCASS AND INTERNAL ORGANS DEVELOPMENT AND GUT HEALTH

Published in AgroLife Scientific Journal, Volume 6, Number 1
Written by Rodica Diana CRISTE, Tatiana Dumitra PANAITE, Cristina TABUC, Mihaela SĂRĂCILĂ, Cristina ȘOICA, Margareta OLTEANU

The study used 96 day-old Cobb 500 chicks, weighed individually and housed in an experimental hall with 320C constant temperature and 23 h light regimen. During the starter stage (1-14 days), all chicks received a conventional diet formulation with sodium monensin (50 g/kg premix). During the growth stage (14-35 days), when the actual feeding trial started, the chicks were weighed, assigned to three groups (32 chicks/group) and housed in the same experimental hall. Throughout the experimental period, the temperature was maintained at 320C, humidity 36%, 23 h light regimen. Like in the starter stage, the chicks had free access to the water and feed. Compared to the diet formulation for the control group (C), conventional formulation, with monensin in the premix, the formulations for the experimental groups included 2% dry oregano (E1), or 2% rosehip powder (E2), but no monensin in the premix. Throughout the experimental period were monitored the bodyweight and the intake. At the end of the feeding trial, 6 chicks from each group were slaughtered in order to make measurements of the relative weight of carcass cuts and internal organs of broilers. Samples of intestinal content were collected for bacteriological assessment (determination of the Enterobacteriaceae, E. coli and Lactic acid bacteria). Throughout the entire experimental period (14-35 days), the average daily feed intake was significantly higher in group C than in groups E1 and E2, but the average daily weight gain and the feed conversion ratio were not different among the three groups. The dietary phytoadditives given the broiler chicks reared under heat stress (320C) had a favourable action in maintaining the health of the intestinal tract, by preserving the balance of the populations of microorganisms colonizing the intestine. Throughout the experimental weeks under heat stress no mortalities were recorded in any of the three groups.

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CRISTE R.D., PANAITE T.D., TABUC C., SĂRĂCILĂ M., ȘOICA C., OLTEANU M. 2017, EFFECT OF OREGANO AND ROSEHIP SUPPLEMENTSON BROILER (14-35 DAYS) PERFORMANCE, CARCASS AND INTERNAL ORGANS DEVELOPMENT AND GUT HEALTH. AgroLife Scientific Journal, Volume 6, Number 1, ISSN 2285-5718, 75-83.

EFFECT OF ROOTING HORMONE TREATMENTS ON PROPAGATION OF Actinidia sp. BY HARDWOOD CUTTINGS

Published in AgroLife Scientific Journal, Volume 5, Number 2
Written by Adrian George PETICILĂ, Roxana Maria MADJAR, Gina VASILE SCĂEȚEANU, Adrian ASĂNICĂ

Recently, kiwifruit consumption increased significantly, having in view its high nutritive and medicinal values. As consequence, the development and expansion of the kiwifruit industry has led to an increasing need for propagation material. The kiwifruit can be efficiently propagated under certain circumstances (proper substrate, hormonal rooting treatments) by hardwood cuttings, the most accessible methods for vegetative propagation for many horticultural crops. Our study presents the results of investigations on the effects of some rhizogene treatments (Radistim, naphtylacetic acid (NAA) 2000 ppm, beta-indolyl butyric acid (IBA) 2000 ppm and a mixture of NAA+IBA 1000 ppm) on root performance of hardwood cuttings for Actinidia deliciosa (AD20, Hayward, Katiuscia, Kramer, Tomuri) and Actinidia arguta (Francesca, Jumbo) species grown on a substrate composed from sand and perlite. It was developed a bifactorial experiment where "a" factor was kiwi cultivar/hybrid and "b" factor was the applied treatment represented by different rhizogene compounds. The efficiency of hardwood cutting under various hormonal rooting treatments was quantified by rooting percentage, number of formed roots, roots lengths (cm/cutting). The best rooting parameters were found when NAA+IBA 1000 ppm solution was used for Actinidia deliciosa (Hayward): rooting percentage was 73.1%, number of formed roots 17.2 and root length was evaluated as 19.3 cm/cutting.

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PETICILĂ A.G., MADJAR R.M., VASILE SCĂEȚEANU G., ASĂNICĂ A. 2016, EFFECT OF ROOTING HORMONE TREATMENTS ON PROPAGATION OF Actinidia sp. BY HARDWOOD CUTTINGS. AgroLife Scientific Journal, Volume 5, Number 2, ISSN 2285-5718, 112-118.

EFFECT OF TIME PROCESSING AT STEPS OF BIOPROCESS SHRIMP WASTE BY THREE MICROBES ON PROTEIN DIGESTIBILITY AND METABOLIZABLE ENERGY PRODUCTS OF NATIVE CHICKEN

Published in AgroLife Scientific Journal, Volume 5, Number 1
Written by Abun, Tuti WIDJASTUTI, Kiki HAETAMI

The shell and head of shrimp is a waste material whose protein content is constrained by its content of chitin (15-20%) making it difficult to digest. The objective of this research was to determine the optimum time required for different microbes bioprocess shrimp waste in order to improve nutrient availability, protein digestibility, and metabolizable energy of these materials in native chickens. Experiments were conducted using a completely randomized design with three microbial treatments and bioprocessing times. Data were statistically analyzed for variance using a Duncan’s Multiple Range test. Shrimp waste materials were treated with each microbe sequentially over time (W) as follows: W1, Bacillus licheniformis (Bl) + Lactobacillus sp. (Ls.) + Saccharomyces cereviseae (Sc), each for 1 d; W2 = Bl+ Ls+ Sc, each for 2 d; W3 = Bl+ Ls+ Sc, each for 3 d. Bioprocessed products of shrimp waste materials were used as a nutrient concentrate in diets of native chickens (crude protein). The optimal crude protein content at W2 of the bioprocessed product was 48.5%, while extract ether, calcium, and phosphorous levels were 7.81%, 7.57%, and 3.14%, respectively. The highest protein digestibility obtained from the best nutrient (W2) with value 72.91%; the best of metabolizable energy of these materials was 2613.90 kcal/kg.

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ABUN, WIDJASTUTI T., HAETAMI K. 2016, EFFECT OF TIME PROCESSING AT STEPS OF BIOPROCESS SHRIMP WASTE BY THREE MICROBES ON PROTEIN DIGESTIBILITY AND METABOLIZABLE ENERGY PRODUCTS OF NATIVE CHICKEN. AgroLife Scientific Journal, Volume 5, Number 1, ISSN 2285-5718, 209-213.

EFFECTS OF BACTERIOCIN AND ORGANIC ACID ON Listeria monocytogenes IN FEED

Published in AgroLife Scientific Journal, Volume 6, Number 1
Written by Ahmet Onder USTUNDAG, Mursel OZDOGAN

Listeria monocytogenes is a Gram-positive, psychrotropic, facultative anaerobic pathogen and it is the etiological agent of listeriosis, a severe foodborne disease of major public health concern. Listeria monocytogenes has high ability to adapt adverse condition. Therefore, the control of Listeria monocytogenes in foods and feeds is very difficult. N this purpose, organic acids and bacteriocins have been used for many years in preservation of foods against pathogen contamination. In this considering, it was aimed to determine the effects of bacteriocin, organic acid, and mixtures of them on the Listeria monocytogenes in this study. Feeds were divided into six experimental groups that consist of control, 150 mg/kg bacteriocin (B150), 300 mg/kg bacteriocin (B300), 3g/kg organic acid (OA), 150 mg/kg bacteriocin + 3 g/kg organic acid (B150+OA) and 300 mg/kg bacteriocin + 3 g/kg organic acid (B300+OA). Listeria monocytogenes cultures were added by spraying on feed samples in sterile bags and were counted by inoculating onto agars at 0, 7, 15, 21 and 28 days. At the end of this study, bacteriocin and organic acid supplementations have been found to have positive effects on Listeria monocytogenes contamination. But the best antilisterial effects was achieved by combined use of bacteriocin and organic acid.

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USTUNDAG A.O., OZDOGAN M. 2017, EFFECTS OF BACTERIOCIN AND ORGANIC ACID ON Listeria monocytogenes IN FEED. AgroLife Scientific Journal, Volume 6, Number 1, ISSN 2285-5718, 262-267.

EFFECTS OF DIFFERENT DOSES AND TYPES OF FERTILIZERS ON GRAZING TIME AND RANGELAND QUALITY

Published in AgroLife Scientific Journal, Volume 5, Number 2
Written by Firat ALATURK, Ahmet GOKKUS

Experimental trails have been conducted between March 2010 and January 2012 in Gerlengec village of Biga District situated in Canakkale Province, Turkey. In this study, control along with 5 kg N/ha 4M (chelate organo-mineral fertilizer, 5-10-0), 10 kg N/ha 4M, 5 kg N/ha composite (20-20-0) and 10 kg N/ha composite of five different types and doses of fertilizer were used. Height of the grass has been increased from 10.0 cm to 110.9 cm and 22.8 cm to 113.1 cm in 2010 and 2011, respectively by increasing the grass layer of the rangeland from 1st March to 30th May. At the same time, the fodder production has been increased from 84.0 kg/ha to 494 kg/ha and 95.8 kg/ha to 631.1 kg/ha in 2010 and 2011, respectively. An increase, both in the grass layer height as well as the fodder production has been accelerated between 10 to 20 April. The application of fertilizers has none significant level of affect to the grazing maturity time of rangeland. The effects of fertilization on legumes in rangeland were important in general while its impact on the species of grains and other related families has mostly been appeared in the months of summer and autumn. According to the two-year average results, legumes that found in plant vegetation were increased by the application of composite fertilizer while decreased due to 4M fertilizer. All of the fertilizers showed an increase in gramineae but reduced the species from other families. All types and doses of fertilizer showed an increase into the quality level of rangeland in each of two years, but an extreme increase of 10 kg N/ha has been occurred in those plots which were applied to 4M fertilizer. According to this, grazing should be started in 20th of April in similar rangelands and 5 kg of nitrogen should be applied to per hectare for improving the quality of rangeland.

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ALATURK F., GOKKUS A. 2016, EFFECTS OF DIFFERENT DOSES AND TYPES OF FERTILIZERS ON GRAZING TIME AND RANGELAND QUALITY. AgroLife Scientific Journal, Volume 5, Number 2, ISSN 2285-5718, 9-14.

EFFECTS OF FERTILIZATION AND ROW SPACING ON GRAIN SORGHUM YIELD GROWN IN SOUTH-EASTERN ROMANIA

Published in AgroLife Scientific Journal, Volume 6, Number 1
Written by Cristina Andreea OPREA, Ciprian BOLOHAN, Doru Ioan MARIN

The paper presents the results of a research conducted during 2014-2016 with the purpose of identifying the optimal interaction of technological factors for the cultivation of grain sorghum (sowing distance x fertilization level), in order to optimize the yield of crops grown in South-East Romania. The research was carried on a soil chromic luvisol using ten fertilization levels and two sowing distances between rows. Results show that both sowing distance and fertilization had a statistically significant influence on sorghum’s grain yield. The most favourable combination of technological factors, which ensured a maximum yield of 9.22 t/ha, was represented by sowing sorghum at the distance of 70 cm between rows and using a fertilization level N120 P60 K60. Compared to the sowing distance of 50 cm between rows, the sowing distance of 70 cm between rows generated yield increases between 0.21 t/ha and 0.48 t/ha.

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OPREA C.A., BOLOHAN C., MARIN D.I. 2017, EFFECTS OF FERTILIZATION AND ROW SPACING ON GRAIN SORGHUM YIELD GROWN IN SOUTH-EASTERN ROMANIA. AgroLife Scientific Journal, Volume 6, Number 1, ISSN 2285-5718, 173-177.

ELECTROCHEMOTHERAPY: A REVIEW OF ITS USE AND LIMITATIONS IN SMALL ANIMAL ONCOLOGY

Published in AgroLife Scientific Journal, Volume 6, Number 1
Written by Ana Maria GOANȚĂ, Lucian IONIȚĂ

Electrochemotherapy (ECT) is a recent anticancer treatment used for solid tumours in which square wave electric pulses are combined with a chemotherapeutic drug administered either intravenously or intratumourally. The drugs most frequently used in veterinary medicine are bleomycin and cisplatin. Due to the advanced cancer stage in which companion animals are usually diagnosed, treatment with surgery alone is either inefficient or not accepted by the owner, either due to loss of function or cosmetic effect. This is where multimodal therapies come in, by combining surgery with chemotherapy, radiation therapy and other therapies. Unfortunately, these are currently not available in our country or are cost-prohibitive. The only major disadvantage of ECT is the need for general anesthesia, especially when the treatment has to be repeated. This paper reviews 13 articles on ECT in small animal medicine so far in order to establish the method’s current indications, limitations and success rates for different types of cancer. Electrochemotherapy has numerous advantages: it is a simple method, with almost insignificant side effects (muscle contractions during the application of electric pulses and in some cases local edema or necrosis after therapy), it can be applied as single therapy or adjuvant to surgery and can be used for inoperable tumours, it can be repeated several times without being less effective and, last but not least, it is an affordable method. In conclusion, ECT is a welcome addition in the fight against cancer in animals as the incidence of diagnosed malignancy in veterinary medicine is ever rising.

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GOANȚĂ A.M., IONIȚĂ L. 2017, ELECTROCHEMOTHERAPY: A REVIEW OF ITS USE AND LIMITATIONS IN SMALL ANIMAL ONCOLOGY. AgroLife Scientific Journal, Volume 6, Number 1, ISSN 2285-5718, 125-132.

Environmental risk assessment of some copper based fungicides according to the requirements of good laboratory practice

Published in Scientific Papers. Series "AgroLife Journal", Vol. 4 ISSUE 1
Written by Marga GRADILA

The paper presents data demonstrating the functionality of biological systems reconstituted with aquatic organisms developed under Good Laboratory Practice testing facility within Research - Development Institute for Plant Protection Bucharest for environmental risk assessment of four fungicides based on copper, according to Good Laboratory Practice requirements. For risk assessment, according to GLP were made the following steps: Good Laboratory Practice test facility was established, we have ensured adequate space for growth, acclimatization and testing for each test species, it was installed a complex water production instalation needed to perform tests, it was achieved control system for checking environmental conditions and have developed specific operating procedures that have been accredited according to Good Laboratory Practice.The results showed that biological systems model of the Good Laboratory Practice test facility in Research - Development Institute for Plant Protection meet the requirements of Organisation for Economic Co-operation and Development Guidelines regarding GLP, and after testing copper-based fungicides in terms of acute toxicity Cyprinus carpio and to Daphnia magna revealed that three of them (copper oxychloride, copper hydroxide and copper sulphate) showed ecological efficiency, ie low toxicity. Metallic copper based fungicides showed a higher toxicity, resulting in fish toxicity symptoms: sleep, sudden immersion, faded, weakness, swimming in spiral, lack of balance, breathing slow and cumbersome, spasms and mortality

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Marga GRADILA 2015, Environmental risk assessment of some copper based fungicides according to the requirements of good laboratory practice. Scientific Papers. Series "AgroLife Journal", Vol. 4 ISSUE 1, PRINT ISSN 2285-5718, 74-78.

EPIDEMIOLOGICAL PROFILE OF RABIESIN PRAHOVA COUNTY, ROMANIA 2010-2015

Published in AgroLife Scientific Journal, Volume 6, Number 2
Written by Vlad VUTA Gheorghe BARBOI, Florica BARBUCEANU, Constantin LUPESCU, Gabriel PREDOI, Viorel SURLARU, Constantin VLAGIOIU

Rabies is a fatal zoonotic viral disease produced by a Lyssavirus and is causing more than 70,000 human deaths each year. Due to the fatality of the disease and in the absence of the specific treatment, vaccination and immunization are the most important methods to fight against rabies. The aim of this study was to evaluate the epidemiological situation of rabies in Prahova County during 2010-2015. During this period in Prahova county from total number of population 0.25%, (n=6513) out of women, respectively 0.28% (n=6739) out of man were immunized against Rabies. 0.32% (n=8324) out of urban population and 0.20% (n=4928) of rural population were immunized against Rabies. In 2010-2015, 63 animals, 51 wild (81%) and 12 domestic (19%) were find positive to Fluorescent Antibody Test (FAT) for Rabies (5 dogs, 6 cats, 1 bear, 1 cattle and 50 fox). Taking into account the number of positive cases in animals as well as the risk of diseases in human population it is strongly recommended to use procedures in place of vaccination for persons who have come into contact with the animal potentially infected with rabies virus.

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VUTA V. BARBOI G., BARBUCEANU F., LUPESCU C., PREDOI G., SURLARU V., VLAGIOIU C. 2017, EPIDEMIOLOGICAL PROFILE OF RABIESIN PRAHOVA COUNTY, ROMANIA 2010-2015. AgroLife Scientific Journal, Volume 6, Number 2, ISSN 2285-5718, 235-238.


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