EVALUATION OF ASEPTIC AND NON-ASEPTIC SYSTEMS AND SUBSTRATE EFFECTS ON SORREL MICROPLANTS CULTIVATED UNDER LED IRRADIATION
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Abstract
This study investigates the impact of aseptic (AS) and non-aseptic (NAS) cultivation systems, using various substrates, on the germination capacity and quality of sorrel microplants. The AS conditions significantly improved germination rates, achieving over 90% capacity compared to lower rates in NAS. Morphological parameters, such as hypocotyl length, were also significantly enhanced under AS. Biochemical analysis showed notable differences in dry matter (9.94±0.97% in AS vs. 6.31±0.14% in NAS), macro- and microelement content, antioxidant activity (up to 11836.28±1065.06 mg TE/100 g FW), total polyphenols (623.01±88.51 mg GAE/100 g FW), and flavonoid levels, highlighting the importance of substrate choice in improving microplant quality. NAS systems exhibited a higher risk of microbial contamination in all substrate types, whereas the AS showed Aspergillus and Penicillium contamination only when organic waste of banana peel was used in high proportions (75 to 100%) as germination substrate. The results suggest that AS, especially with 100% agar substrate (MV2), offers a more effective and safer method for producing high-quality, edible sorrel microplants.
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