THE MANAGEMENT OF ERYTHROCYTE MEMBRANE PERMEABILITY IN RATS FED ON CHOLESTEROL RICH DIET AND THE ROLE OF THE 1H NMR METHOD

Abstract

The aim of this study was related with the investigation of erythrocyte membrane permeability of rats fed on rich cholesterol diet by means of the 1HNMR technique. Three groups of 32 months old rats received rich cholesterol diet for 6 weeks combined with Aslavital or Procaine (4 mg/kg body weight). Group (A) Control. The animals fed on cholesterol rich diet were divided into 3 groups: group (B) - hypercholesterolemic diet, group (C) also with Aslavital i.m. (4 mg/kg corp); group (D) - also with Procaine treatment (4 mg/kg corp) to test its properties as an antiatherosclerotic drug. Our data pointed out a decrease of proton half time within erythrocite which accounts for an accelerated proton exchange in all groups of rats with high level of serum cholesterol. In controls the exchange of water through red blood cell membrane is accelerated in parallel with increase in local temperature. Red blood cell membrane permeability towards water can be accounted as an index of cardiovascular system recovery, important in maintaining a dynamic balance with vascular destruction due to high blood pressure.