SCREENING AMONG MICRO AND MACROMYCETES FOR LACCASE PRODUCTION

Abstract

Laccases show multiple biotechnological application and different fungal groups have been widely reported as laccase producers. The main aim of our research it was to perform on-plate screening for laccase production among different macro and micromycetes, while optimising the screening protocol in the presence of different guaiacol concentrations. From our collection were taken into account filamentous fungi belonging to the following species: Aspergillus clavatus, A. aculeatus, Botrytis cinerea, Neurospora crassa, Trichoderma sp., Penicillium digitatum. Among the macromycetes were tested Laetiporus sulphureus, Ganoderma lucidum, Agaricus bisporus and Pleurotus ostreatus. The screening was performed on PDA added with guaiacol. The positive microorganisms belong to two strains of Trichoderma spp. isolated from soils, one variety of P. ostreatus and two of A. bisporus originating from supermarket wastes. In the case of P. ostreatus, the use of guaiacol higher than 0.1% has inhibited the fungal growth, as well as the halo-formation. In the case of Trichoderma spp. strains the use of guaiacol above 0.1% and till 1% dind’t lead to any halo formation, while the mycelial growth was not inhibited; relevant halos were registered for the concentrations of 0.01% and 0.025%, when the maximum was reached after 6-7 cultivation days.