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The paper aimed to present the Physiological Tolerance Test of Combination Treatment of Antigen-G and Curcumin Extract on VEGF Levels, Mortality Rate of BALB/c. This research consists of several stages. The TMd-G antigen (ITMd) was isolated from the thoracic segments of Musca domestica larvae 4th instar stage and the thoracic segments of the adult cycle stage, 5 days old. The larvae were obtained from the rearing process using defined media. Curcumine extract (EK) was obtained from Curcuma xanthorrhiza rhizomes through the process of broyage, mixing, precipitation, lyophilization and collection of dry EK. The VEGF serum molecules were significant higher (P<0.05) in Ek20Ag3 to Ek20Ag9 then in other combination treatment. The zero-mortality rate obtained in using AgLMd.