ANALYSIS OF THE INFLUENCE OF HYDROLYZED COLLAGEN AND THYME OIL ON THE GERMINATION OF BRASSICA NAPUS SEEDS AND THEIR USE IN CONTROLLING ALTERNARIA BRASSICAE

Abstract

This study presents some data regarding the treatment of rape seeds with hydrolyzed collagen and thyme oil in different concentrations, in order to assess germination. Moreover, in order to evaluate the in vitro activity of collagen combined with antifungal substances on the seeds of Brassica napus infected with Alternaria brassicae, this paper highlights the importance of using alternative non-polluting methods as essential oils in plant pathogens management.

A control group (untreated) and experimental (pre-treated and treated) lots were used, with the observations focused on the development of the pathogen, as well on the occurrence of the necrosis symptoms.

Overall, three in vitro experiments were performed, as follows: seed germination, fungus prevention and infected seed treatment. In terms of growing, collagen alone did not delayed the germination of the rapeseed. On the other hand, the mixture of collagen (stock solution and 50%) and thyme oil (0.5 ml, 0.25 ml respectively) inhibited germination. As for the preventive stage, the mixture of collagen and thyme oil 1 ml remained on the seed surface, preventing the infection of B. napus. Finally, the treatment of infected seeds with collagen and thyme oil 0.1 ml reduced the attack of the pathogen which was evidenced by the slight occurrence of necrotic radicles and hypocotyl in rapeseed.