Main Article Content
Cryopreservation (storage at -196°C) is one of the techniques used for ex situ conservation to guarantee the long-term
storage of different biological materials. Polypodium vulgare (Common polypody) is an evergreen fern used as food
and medicine. To our knowledge, no cryopreservation protocol for gametophytes from this species is available. In this
context, our paper aimed to screen a suitable cryopreservation protocol for P. vulgare - an important source of
secondary metabolites. Different protocols were tested to identify a suitable cryopreservation protocol. Techniques like
light microscopy and biochemical assays were used to certify an appropriate protocol for this fern. Gametophyte
desiccation for 3 hours in the sterilized airflow before rapid immersion in liquid nitrogen provides sufficient
dehydration to assure a good rate of survival.
This work is licensed under a Creative Commons Attribution 4.0 International License.