Published in AgroLife Scientific Journal, Volume 5, Number 2
Written by Mihaela-Carmen EREMIA, Irina LUPESCU, Gabriela Valeria SAVOIU, Maria SPIRIDON
The trypsin inhibitor from common flax seeds (Linum usitatissimum trypsin inhibitor, abbreviate LUTI) is a member of the potato inhibitor I family. This was isolated and purified by ethanol fractionation, ion exchange chromatography and affinity chromatography. To determine the molecular mass of protease inhibitor we utilized gel electrophoresis in denatured conditions (SDS-PAGE). LUTI is the first serine protease-type inhibitor isolated from a plant of Linaceae family, and has a molecular mass 7000D.
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