PRINT ISSN 2285-5718, CD-ROM ISSN 2285-5726, ISSN ONLINE 2286-0126, ISSN-L 2285-5718


Published in AgroLife Scientific Journal, Volume 11, Number 1
Written by Alexandra OPRESCU, Aurel VASIU, Carmen Dana ȘANDRU, Diana OLAH, Marina SPÎNU, Emoke PALL, Gheorghiță DUCA, Florina MARIAN, Sergiu ZĂBLĂU, Ioan Liviu MITREA

Microphages represent the first line of defence against microbial aggression. The research aimed at evaluating phagocytosis during a Marek’s disease episode in Rosso layers, showing ocular lesions, in spite of the vaccination at 1 day of age. Heparinized blood (50 IU/ml) was subjected to the in vitro carbon particle inclusion test on days 0 and 15 of the episode. Two microliters of an India ink were added to 0.5ml of blood; 0.15·ml of the mixture were transferred to 2·ml of saline and the rest was incubated for 30·min at 37°C and procedure repeated. Phagocytic activity index was calculated as the difference between the natural logarithms of the optical densities of the phagocytosis read spectrophotometrically (λ = 535·nm, d = 1·cm) after centrifugation (2500rpm for 10min), divided by time. The values indicated a significant (p = 0.0259) increase of the phagocytosis towards the end of the monitoring interval, thus indicating an evolving disease and underlining the probability of innate immunity being stimulated by the herpesvirus during the observation period. Specific cell-mediated immunity and functional levels of humoral immune effectors quantification could complete the pathogenic pattern in these birds.

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