Published in AgroLife Scientific Journal, Volume 11, Number 2
Written by Mark SHAMTSYAN, Boris KOLESNIKOV, Sergey SOROKIN
In this research the screening of several higher fungi - basidiomycetes cultures for the presence of collagenolytic activity was carried out. The highest collagenase activity was detected in submerged culture of higher fungus Coprinus lagopides. The enzyme preparation of collagenase was isolated from culture liquid of this producer. The optimum temperature and pH of the collagenolytic activity of the enzyme preparation were determined. The selection of nitrogen and carbon sources and ratios of carbon and nitrogen sources in the composition of nutrient mediums was carried out in order to increase the collagenase activity of fungus submerged culture. In addition to the collagenase activity of producers culture the amount of accumulated biomass, pH and protein concentration in the culture liquid of basidiomycete were also determined.
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